FIG. 5.

Severalfold change in the levels of carbonylated proteins in wild-type (MG1655Δlac) cells and in cells lacking the proteases Lon (ÅF66), HslVU (ÅF49), and ClpPX (PhB1907), ClpPX and Lon (PhB1465), and ClpPX, Lon, and HslVU (PhB1466). Protein extracts were from exponentially growing cells (open bars) and cells from an overnight stationary phase sample (grey bars). Carbonyl levels were determined by a slot-blot immunoassay and quantified using Image Gauge software. All levels were related to that obtained for the wild type during exponential growth, which was assigned a value of 1.0.