TABLE 2.

Proteins subjected to stasis-induced carbonylation at the time when carbonyl content is maximal. Proteins in bold have been demonstrated to be substrates for DnaK (19).

Protein no.	Gene	Size (kDa)	Protein name
1	rpoB	151	RNA polymerase β-subunita
2	purL	141	Phosphoribosyl formylglycinamide synthase
3	carA	119	Carbamoylphosphate synthase
4	aceE	100	E1 component of pyruvate dehydrogenase
5	metE	85	Tetrahydropteroyltriglutamate methyltransferase
6	fus	78	Protein chain elongation factor G
7	dnaK	69	Molecular chaperone DnaK
8	aceF	66	E2 component of pyruvate dehydrogenase
9	ptsI	64	Phosphoenolpyruvate-protein phosphotransferase
10		61	Polyamine-induced protein precursor
11	groEL	57	Molecular chaperone GroEL
12	glnA	52	Glutamine synthetase
13	gltD	52	Glutamate synthase
14	pyk	51	Pyruvate kinase
15	gnd	49	6-Phosphogluconate dehydrogenase
16	icd	46	Isocitrate dehydrogenase
17	glyA	46	Serine hydroxymethyltransferase
18	serA	44	d-3-phosphoglycerate dehydrogenase
19	sucB	44	Dihydrolipoamide succinyltransferase
20	fabB	43	β-Keto-[acyl-carrier-protein] synthetase
21	tufA	42	Protein chain elongation factor Tu
22	sucC	41	Succinyl CoA ligase
23	znuA	34	High-affinity zinc uptake system protein precursor
24	mdh	32	Malate dehydrogenase

^aProteins that have been demonstrated to be substrates for DnaK (19) are shown in boldface characters.