TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Genotype, serotype, or description | Reference or source |
|---|---|---|
| E. coli | ||
| BL21[λ DE3] | F−ompT hsdSB(rB−mB−) gal dcm [λ DE3] | Novagen |
| DH5α | K-12 φ80d deoR lacZΔM15 endA1 recA1 hsdR17(rK−mK+) supE44 thi-1 gyrA96 relA1 Δ(lacZYA-argF)U169 F− | 54 |
| DH5α[λ pir] | K-12 φ80d deoR lacZΔM15 endA1 recA1 hsdR17(rK−mK+) supE44 thi-1 gyrA96 relA1 Δ(lacZYA-argF)U169 F− [λ pir] | 9 |
| SM10[λ pir] | K-12 thi-1 thr-1 leuB6 tonA21 lacY1 supE44 glnV44 recA::RP4-2-Tc::Mu [λ pir] | 34 |
| S. enterica Serovar Typhimurium LT2 SL1306 | galE503; R-LPS | SGSC163a |
| K. pneumoniae | ||
| CWK2 | O1:K−; derivative of CWK1 (O1:K20); Strr Apr | 68 |
| CWG631 | galE::pRE112 derivative of CWK2; Cmr | This study |
| Plasmids | ||
| pBAD18-Km | Arabinose-inducible expression vector; Kmr | 17 |
| pET28a(+) | IPTG-inducible expression vector; Kmr | Novagen |
| pRE112 | Mobilizable suicide vector used for chromosomal insertions requiring λpir for replication; Cmr | 9 |
| pWQ67 | pET28a(+)-derivative expressing GlaKP with an N-terminal His6-tag; Kmr | This study |
| pWQ69 | pBAD18-Km-derivative expressing His6-GlaKP; Kmr | This study |
| pWQ72 | pBAD18-Km-derivative expressing GalE; Kmr | This study |
| pWQ73 | pRE112-derivative containing an internal fragment of galE, used to construct CWG631; Cmr | This study |
a
Salmonella Genetic Stock Center.