TABLE 1.
Susceptibility of V. cholerae strains carrying different combinations of MSHA and TCP genes to a genetically marked derivative of KSF-1φa
| Strain | Description | Presence of
|
Susceptibility to KSF1-Kmφ | |
|---|---|---|---|---|
| mshA | tcpA | |||
| AK-31047 | O1 | + | + | 5.4 × 10−3 |
| C6706 | O1 | + | + | 3.2 × 10−2 |
| KHT46 | C6706ΔmshA | − | + | 0 |
| AL-11089 | O139 | + | + | 2.3 × 10−2 |
| Env-99 | O139 | + | + | 7.5 × 10−3 |
| Env-002 | O1 | + | + | 6.3 × 10−3 |
| O395 | O1 | + | + | 2.7 × 10−5 |
| TCP-2 | O395 ΔtcpA ΔctxA | + | − | 2.1 × 10−5 |
| 55V71 | Non-O1, non-O139 | + | − | 4.2 × 10−2 |
| 761V1325 | Non-O1, non-O139 | + | − | 3.4 × 10−2 |
| 775V1346 | Non-O1, non-O139 | + | − | 6.2 × 10−2 |
| 825V1409 | Non-O1, non-O139 | − | − | 0 |
| 824V1406 | Non-O1, non-O139 | − | − | 0 |
| 828V1412 | Non-O1, non-O139 | − | − | 0 |
| 819V1399 | Non-O1, non-O139 | + | − | 5.7 × 10−2 |
| 820V1401 | Non-O1, non-O139 | + | − | 6.3 × 10−2 |
| 883V1486 | Non-O1, non-O139 | + | − | 9.9 × 10−3 |
| 886V1491 | Non-O1, non-O139 | + | − | 9.2 × 10−3 |
a
Presence of different genes was detected using DNA probes and PCR assays. Susceptibility to the phage was determined using a genetically marked phage (see text for details). The values are the proportions of total cells infected (number of colonies growing on kanamycin plates divided by the total number of colonies recovered). Values are averages from three independent assays.