Figure 5. Similar frequencies and numbers of wild-type (WT) and NCOR1-cKO Treg cells within small intestinal intraepithelial lymphocyte (SI-IEL) and lamina propria cell (SI-LP) populations in adoptive CD4⁺ T cell transfer colitis.

(a) Flow cytometric analysis of SI-LP cells isolated from recipient Rag2^–/– mice injected with WT CD45.1⁺CD4⁺ T cells only (top panel) or with WT CD45.1⁺CD4⁺ T cells together with either WT (middle panel) or NCOR1-cKO Treg cells (lower panel). Plots shows the percentage of viable CD4⁺, CD45.1⁺FOXP3^– and CD45.1^–FOXP3⁺ cells. (b) Percentages (upper panel) and numbers (lower panel) of CD45.2⁺FOXP3⁺ cells from SI-IEL, SI-LP, spleen, and mLNs of recipient Rag2^–/– mice injected with WT CD45.1⁺CD4⁺ T cells together with either CD45.2⁺ WT or NCOR1-cKO Treg cells. (c) Percentages (upper panel) and numbers (lower panel) of CD4⁺CD45.1⁺ cells from SI-IEL, SI-LP, spleen, and mLNs of Rag2^–/– mice injected with either WT Treg cells, NCOR1-cKO Treg cells, or injected with naïve CD4⁺ T cells only. (a) Numbers indicate the percentages of cells in the respective gate. (b,c) Each symbol indicates one mouse. Horizontal bars indicate the mean. *p<0.05, **p<0.01, and ***p<0.001. (b) Unpaired two-tailed Student’s t-test or (c) one-way ANOVA analysis followed by Tukey’s multiple-comparisons test. Data are representative (a) or show a summary (b,c) of at least eight mice (except control groups with six mice) that were analyzed in three independent experiments.

Figure 5—figure supplement 1. IFNγ and IL17A expression in CD4⁺ T cells co-transferred with either wild-type (WT) of NCOR1-cKO Treg cells.

(a) Flow cytometric analysis showing IFNγ and IL17A expression in CD45.1⁺ CD4⁺ T cells isolated from small intestinal intraepithelial lymphocytes (SI-IEL), small intestinal lamina propria cells (SI-LP), spleen and mLNs. (b) Summary of all experiments as described in (a). (a,b) Cells were pre-gated on CD4⁺ T cells. (a) Numbers indicate the percentages of cells in the respective quadrants. (b) Each symbol indicates one mouse. Horizontal bars indicate the mean. *p<0.05, **p<0.01, and ***p<0.001; one-way ANOVA analysis followed by Tukey’s multiple-comparisons test. Data are representative (a) or show the summary (b) of at least six mice (except CD4⁺ T cells only controls with at least four mice) that were analyzed in three independent experiments.