TABLE 1.
Summary of purification of Pz peptidases A and B
| Purification step | Total protein (mg) | Sp act (U/mg protein) | Fold | Total activity (U) | Yield (%) |
|---|---|---|---|---|---|
| Pz peptidase A | |||||
| 1. Cell-free extract | 7,370 | 0.045 | 1 | 332 | 100 |
| 2. Ammonium sulfate fractionation | 2,120 | 0.101 | 2.24 | 214 | 64.5 |
| 3. DEAE cellulose | 1,150 | 0.161 | 3.58 | 185 | 55.7 |
| 4. Butyl-TOYOPEARL 650S | 91.5 | 0.798 | 17.7 | 73.0 | 22.0 |
| 5. 1st Sephadex G-200 | 11.8 | 4.91 | 109 | 57.9 | 17.4 |
| 6. 2nd Sephadex G-200 | 4.42 | 9.76 | 217 | 43.1 | 13.0 |
| 7. DEAE Sepharose CL-6B | 1.66 | 18.4 | 409 | 30.5 | 9.2 |
| 8. 3rd Sephadex G-200 | 0.92 | 24.7 | 549 | 22.7 | 6.8 |
| Pz peptidase B | |||||
| 1. Cell-free extract | 5,890 | 0.0477 | 1 | 281 | 100 |
| 2. (NH4)2SO4 fractionation | 3,230 | 0.0845 | 1.77 | 273 | 97.2 |
| 3. DEAE cellulose | 1,530 | 0.144 | 3.01 | 220 | 78.3 |
| 4. DEAE Sepharose CL-6B | 225 | 0.267 | 5.60 | 60.0 | 21.3 |
| 5. 2nd DEAE Sepharose CL-6B | 94.9 | 0.363 | 7.09 | 34.5 | 12.3 |
| 6. Sephadex G-200 | 67.9 | 0.481 | 10.1 | 32.7 | 11.6 |
| 7. Butyl-TOYOPEARL 650S | 29.3 | 0.345 | 7.23 | 10.1 | 3.6 |
| 8. Phenyl Sepharose CL-4B | 11.2 | 0.730 | 15.3 | 8.18 | 2.9 |
| 9. Ethyl agarose | 5.93 | 1.45 | 30.4 | 8.60 | 3.1 |
| 10. Sephacryl S-300 | 2.35 | 2.32 | 48.6 | 5.45 | 1.9 |