TABLE 1.

Oligonucleotides used in this study

Primer	Primer sequence (5′ to 3′)a	Purpose of mutation(s)
PMM PET FOR	catttatagGaTCCtatgggtaagttgtttggaactg	BamHI
PMM PET REV	cctataAGCTTtactagttcactcactaacttttcc	HindIII
PS309N FOR	gtggactaaggtaggtaAcatagatattgcgc	S309N
PS309N REV	gcgcaatatctatgTtacctaccttagtccac	S309N
PS309A FOR	gtggactaaggtaggtGCcatagatattgcgc	S309A
PS309A REV	gcgcaatatctatgGCacctaccttagtccac	S309A
PMMS2Q FOR	gtggactaaggtaggtCAGatagatattgcgc	S309Q
PMMS2Q REV	gcgcaatatctatCTGacctaccttagtccac	S309Q
PMMS2V FOR	gtggactaaggtaggtGTGatagatattgcgc	S309V
PMMS2V REV	gcgcaatatctatCACacctaccttagtccac	S309V
PMMS2T FOR	gtggactaaggtaggtaCcatagatattgcgc	S309T
PMMS2T REV	gcgcaatatctatgGtacctaccttagtccac	S309T
PMMS2D FOR	gtggactaaggtaggtGAcatagatattgcgc	S309D
PMMS2D REV	gcgcaatatctatgTCacctaccttagtccac	S309D

^aUppercase letters indicate base substitutions to create restriction sites or mutations.