TABLE 2.

Kinetic values for rSsoPHM and mutagenically altered variants thereof^a

Variant	Km [G-6-P], mMa	Vmax (μmol G-1-P/min/mg)	% of WTb
rSsoPHM	13	0.45	100
S309A	9	0.26	58
S309T	8	0.16	36
S309V	12	0.16	36
S309N	8	0.12	27
S309Q	6	0.10	22
S309D	2	0.02	4

^aShown are the Michaelis constants toward α-d-glucose 6-phosphate [G-6-P] for rSsoPHM and mutagenically altered variants in which Ser-309 has been replaced by the indicated residues.

^bWT, wild type.