UV relative absorbance versus temperature curves for three dsDNAs in two adding modes and various molar ratios. Column I indicates that S2, S4 and S6 are guest strands and added into host strands S1, S3 and S5, respectively. Column II shows an inverse-adding mode to Column I. Plots for various molar ratios of 20:1 (squares), 10:1 (circles), 5:1 (triangles), 2.5:1 (diamonds) and 1:1 (crosses).