. 2000 Dec 1;28(23):4762–4768. doi: 10.1093/nar/28.23.4762

Table 1. Hyperchromicity factors for six ssDNAs and the resulting dsDNAs derived from linear fitting of 1/h_m versus 1/[1 + (m – 1)x_i] in two adding modes.

Strand	Adding mode	h_i^a	h_D^b	Average h_D^c	Y^d (%)
S1S2	S2 plus various S1	h_S1 = 1.017	1.123	1.130	0.991
	S1 plus various S2	h_S2 = 1.062	1.136
S3S4	S4 plus various S3	h_S3 = 1.112	1.224	1.216	0.945
	S3 plus various S4	h_S4 = 1.077	1.208
S5S6	S6 plus various S5	h_S5 = 1.137	1.242	1.241	0.919
	S5 plus various S6	h_S6 = 1.119	1.239

^ah_i indicates the hyperchromicity factor of ssDNAs and was calculated by the intercept of a linear fitting of 1/h_m versus 1/[1 + (m – 1)x_i] (i = A or B).

^bh_D indicates hyperchromicity factor of the resulting dsDNAs and was determined by the slope of a linear fitting in two adding modes.

^cAverage h_D indicates the average hyperchromicity factor of the two adding modes and relative deviations of S1S2, S3S4 and S5S6 are 1.15, 1.32 and 0.242%, respectively.

^dY is the extent of duplex association and can be calculated by equation 10.

Table 1. Hyperchromicity factors for six ssDNAs and the resulting dsDNAs derived from linear fitting of 1/hm versus 1/[1 + (m – 1)xi] in two adding modes.

Table 1. Hyperchromicity factors for six ssDNAs and the resulting dsDNAs derived from linear fitting of 1/h_m versus 1/[1 + (m – 1)x_i] in two adding modes.