Table 2. van’t Hoff thermodynamic parameters for three dsDNAs derived from UV measurementa.
| DNA | Tmb (°C) | Non-two-state transition | Average curve fitting | ||||
|---|---|---|---|---|---|---|---|
| Sequence | –ΔH (kcal/mol) | –ΔS (eu) | –ΔG37c (kcal/mol) | –ΔH (kcal/mol) | –ΔS (eu) | –ΔG37c (kcal/mol) | |
| S1S2 | 45.2 | 64.4 ± 1.6 | 175.3 ± 5.1 | 10.0 ± 0.06 | 62.2 ± 2.1 | 169.6 ± 6.7 | 9.6 ± 0.17 |
| S3S4 | 65.8 | 105.9 ± 2.9 | 285.7 ± 8.8 | 17.3 ± 0.17 | 108.4 ± 1.6 | 292.7 ± 5.1 | 17.6 ± 0.02 |
| S5S6 | 75.8 | 144.8 ± 3.0 | 388.5 ± 9.7 | 24.3 ± 0.03 | 147.9 ± 2.5 | 396.1 ± 7.5 | 25.0 ± 0.12 |
aAll experiments were carried out in a buffer solution containing 1 M NaCl/10 mM Na2HPO4 /1 mM Na2EDTA (pH 7.0).
bMelting temperature were determined at 5 µM total species concentration.
cFree energy of a duplex formation at 37°C.