TABLE 3.

Frameshifting efficiencies on AGA_AGA in an emrK context under different expression conditions

Plasmid/strain	Reporter copy no.	Promoter	Inducer	β-Galactosidase activity (Miller units)a		Average frameshifting level (%) ± SD
				Frameshift reporter	In-frame control
pGHM-emrK/DH5α	15-20	Ptac	1 mM IPTG	NA	NA	7.4 ± 3.4
pSKAGS-emrK/CP79	15-20	Ptac	None	41 ± 2	1,847 ± 105	2.2 ± 0.2
			1 mM IPTG	376 ± 33	4,698 ± 545	8.0 ± 1.2
pSKAGS-emrK/CP78	15-20	Ptac	None	10 ± 1	690 ± 67	1.5 ± 0.1
			1 mM IPTG	63 ± 6	4,559 ± 348	1.4 ± 0.2
pSKAGS-emrK/BW27786	15-20	Ptac	None	20 ± 2	1,449 ± 160	1.4 ± 0.2
			1 mM IPTG	345 ± 55	8,850 ± 465	3.9 ± 0.7
JFG001 (derived from BW27786)	1	ParaB	0.02% arabinose	1.6 ± 0.1	165 ± 15	1.0 ± 0.1
			0.2% arabinose	2.6 ± 0.3	287 ± 13	0.9 ± 0.1

^aNA, not applicable.