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. 2005 Jun;187(12):3990–3996. doi: 10.1128/JB.187.12.3990-3996.2005

FIG. 2.

FIG. 2.

Phenotype of nar::kat mutants. A) Western blot for detecting NarG in soluble (S) and particulate (P) fractions of nitrate/anoxia-induced cultures of the wild-type strain (Wt) and its three nar::kat derivatives (narC, narJ, narI). B) The presence of NarG is analyzed in the soluble (S) and particulate (P) fractions from a narC::kat mutant (narC) and from a derived strain complemented with a wild-type narC gene (narC/pIWnarC) by transformation with plasmid pIWnarC. The enzyme activities (nmol of nitrite/min and OD550 unit) from each fraction are indicated.