TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s) | Source or reference |
|---|---|---|
| Strains | ||
| E. coli | ||
| DH5α | φ80 lacZΔM15 ΔlacU169 recA1 endA1 hsdR17 supE44 thi-1 gyrA relA1 | 16 |
| JM83 | ara Δlac-pro strA thi φ80 lacZΔM15 | 36 |
| M. xanthus | ||
| DK1622 | Wild type | 20 |
| DK4294 | Tn5 lac (Kmr) Ω4406 | 28 |
| DK4506 | Tn5 lac (Kmr) Ω4506 | 28 |
| MMF107, -108, -109 | attB::pREG4406 | This work |
| MJL100 | Tn5 lac (Tcr) Ω4406 | This work |
| MJL123-1, -5, -7, -8, -9 | Tn5 lac (Tcr) Ω4406 Ω4406::pJL23 | This work |
| MPV4406-3.2 | attB::pPV4406-3.2 | This work |
| MPV4406-2.4 | attB::pPV04406-2.4 | This work |
| MPV4406-1.8 | attB::pPV04406-1.8 | This work |
| MPV4406-1.0 | attB::pPV04406-1.0 | This work |
| MPV1.8-1175 | Ω4406::pPV1.8-1175 | This work |
| MPV2.4-1175 | Ω4406::pPV2.4-1175 | This work |
| MPV4406-2.4SC | attB::pPV04406-2.4SC | This work |
| MPV0.74kbins | Tn5 lac (Tcr) Ω4406 orf2::pPV0.74kbins | This work |
| LS203 | csgA653 | 49 |
| MPV1.8C | csgA653 attB::pPV04406-1.8 | This work |
| MPV1.0C | csgA653 attB::pPV04406-1.0 | This work |
| Plasmids | ||
| pGEM-7Zf | Aprlacα | Promega |
| pGEM4406 | Apr (pGEM-7Zf)a; 12-kb XhoI fragment from DK4294 | This work |
| pREG1666/pREG1727 | Apr Kmr P1-inc attP ′lacZ | 11 |
| pREG4406 | Apr Kmr (pREG1666); 3.2-kb XhoI-BamHI fragment from pGEM4406 | This work |
| pJL30 | Apr (pGEM-7Zf); 3.2-kb XhoI-BamHI fragment from pGEM4406 | This work |
| pREG429 | Apr Kmr P1-inc | 13 |
| pJL23 | Apr Kmr (pREG429); 3.2-kb ClaI-BamHI fragment from pJL30 | This work |
| pUC19 | Aprlacα | 61 |
| pJL40 | Apr (pUC19); 1.0-kb SalI-SalI fragment from pJL30 | This work |
| pBR322 | Apr Tcr | 3 |
| pKV322m | Apr Tcr (pBR322); a C to T mutation eliminated the BamHI site in pBR322 | This work |
| pKV322mlacZ | Apr Tcr (pKV322m); 3′ end of lacZ preceded by a unique EcoRI site | This work |
| pPV12K | Apr Tcr (pKV322mlacZ); 7.6-kb EcoRI fragment containing attP ′lacZ from pREG1727 | This work |
| pPV4406-3.2 | Apr Tcr (pPV12K); 3.2-kb XhoI-BamHI fragment from pJL30 | This work |
| pCR2.1-TOPO | Apr Kmrlacα | Invitrogen |
| pPV4406-2.4 | Apr Kmr (pCR2.1-TOPO); 2.4-kb Ω4406 upstream DNA segment generated by PCR | This work |
| pPV04406-2.4 | Apr Tcr (pPV12K); 2.4-kb XhoI-BamHI fragment from pPV4406-2.4 | This work |
| pPV4406-1.8 | Apr Kmr (pCR2.1-TOPO); 1.8-kb Ω4406 upstream DNA segment generated by PCR | This work |
| pPV04406-1.8 | Apr Tcr (pPV12K); 1.8-kb XhoI-BamHI fragment from pPV4406-1.8 | This work |
| pPV4406-1.0 | Apr Kmr (pCR2.1-TOPO); 1.0-kb Ω4406 upstream DNA segment generated by PCR | This work |
| pPV04406-1.0 | Apr Tcr (pPV12K); 1.0-kb XhoI-BamHI fragment from pPV4406-1.0 | This work |
| pREG1175 | Apr Kmr P1-inc ′lacZ | 12 |
| pPV1.8-1175 | Apr Kmr (pREG1175); 1.8-kb XhoI-BamHI fragment from pPV04406-1.8 | This work |
| pPV2.4-1175 | Apr Kmr (pREG1175); 2.4-kb XhoI-BamHI fragment from pPV4406-2.4 | This work |
| pPV4406-2.4SC | Apr Kmr (pPV4406-2.4); a GTG to TGA mutation changed the putative start codon of orf2 to a stop codon | This work |
| pPV04406-2.4SC | Apr Tcr (pPV12K); 2.4-kb XhoI-BamHI fragment from pPV4406-2.4SC | This work |
| pPV0.74kbins | Apr Kmr (pCR2.1-TOPO); 747-bp segment of orf2 generated by PCR | This work |
a
The vector is indicated in parentheses.