FIG. 3.

Identification of replication and partition functions of bacteriophage LE1. A. The ability of each plasmid construct to yield L. biflexa transformants and the stability of the plasmid without selective pressure are indicated on the right. ≪-≫ indicates that the plasmid was not replicative in L. biflexa. Plasmid stability in L. biflexa was evaluated as described in Material and Methods. The primers used to generate each DNA fragment are indicated. Boxes indicate site-directed mutagenesis of direct repeats (DR) and inverted repeats (IR), and nucleotide substitutions are written in uppercase. ND, not determined. B. Sequence alignment of Burkholderia cepacia phage Bcep22 protein (Bcep2), Lactococcus lactis phage phi31.1 (phi31), Lactococcus phage BK5-T (BK5), Salmonella enterica serovar Typhimurium phage ST64B (ST64B), Shigella flexneri phage V (phage V), and L. biflexa phage LE1 Rep (LE1) proteins. Residues conserved in at least four homologous proteins are shaded.