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Analysis of expression driven by rho regulatory regions in the presence of bicyclomycin. Expression was determined from NA1000 cells harboring the respective constructs described in Fig. 3 after incubation at 30°C for 3 h in the presence or absence of 100 μg/ml bicyclomycin. The β-galactosidase (β-gal) activities are expressed in Miller units (21) and are the averages of results of at least three independent assays.
