FIG. 5.

RNA electrophoretic mobility shift assay of rho mRNA. The indicated amount of purified Rho was incubated with 70 ng of ³²P-labeled RNA fragment for 20 min, and the complexes were resolved in a 5% polyacrylamide gel. Lane 1, RNA probe; lanes 2 to 5, increasing amounts of Rho (0.5, 1, 3, and 5 μg, respectively) added to the RNA probe; lane 6, the probe incubated with 3 μg of Rho and 0.7 μg of the same nonradioactive RNA competitor; lane 7, the probe incubated with 3 μg of Rho and 1 μg of tRNA; lane 8, the probe incubated with bovine serum albumin (5 μg).