FIG. 1.

(A, C, and D) Cytochrome c profiles of representatives from three classes of B. pertussis Tn5 mutants deficient in cytochrome c oxidase activity. Heme staining was carried out on B-PER extracts from the indicated strains. Equivalent amounts of protein (approximately 20 μg) were loaded in each lane, shown stained in panel B, which represents a duplication of the strains and conditions shown in panel A. The concentration of DTT used for cells grown overnight in cultures is designated above each lane number (A, C, and D). The location of full-length CtaC as well as a heme-containing truncated CtaC (CtaC*: see text) is indicated (D).