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. 2005 Jun;187(12):3941–3949. doi: 10.1128/JB.187.12.3941-3949.2005

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Copyright © 2005, American Society for Microbiology

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FIG. 3. — Western blot analysis using CcsB antisera of the indicated B. pertussis protein fractions and strains. CcsB antisera generated against the 50-kDa periplasmic domain (P2) of the B. pertussis CcsB protein reacted with the hexahistidine-tagged P2 antigen (lanes 7 to 9). Extracts in lanes 1 to 5 were from crude sonicated cells of the indicated B. pertussis strains. A B-PER extract from the WT strain BC39 is shown in lane 6. Membrane and soluble fractions, as described in the text, are shown in lanes 10 to 24.