TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s)a | Source or reference |
|---|---|---|
| Strains | ||
| B. pertussis | ||
| BC39 | Wild type; TOHAMA III background; Nalr Smr | 9, 10 |
| RGK343 | BC39 dsbB::pRGK340; Nalr Smr Kanr | This study |
| Tn5 mutants | See Table 2 | This study |
| E. coli | ||
| S17-1 | λ pir conjugation mobilizing strain | Laboratory collection |
| TB1 | General cloning vector | Laboratory collection |
| CC118 | Pho− strain | 30 |
| Tuner (DE3) | T7 expression host | Novagen |
| Plasmids | ||
| pET2-Blue | T7 expression vector | Novagen |
| pTGN | Mini-Tn5gfp-km transposon; Kanr | 58 |
| pss2141 | Suicide vector for homologous recombination; Gmr Sms | 56 |
| pUC119 | Cloning vector; Ampr | Laboratory collection |
| pUC4-KIXX | Aminoglycoside phosphotransferase Kanr cassette | Pharmacia |
| pRGK309 | CcsB:PhoA expression vector; Tetr | 3 |
| pRGK338 | puc119-DsbB; 2,469-bp PCR product including dsbB ORF as HinDIII fragment in puc119; Ampr | This study |
| pRGK339 | dsbB::Kanr; pRGK338 with Kanr cassette from pUC4-KIXX replacing dsbB ORF; Ampr Kanr | This study |
| pRGK340 | dsbB::Kanr HinDIII fragment from pRGK339 in pss2141; Gmr Kanr Sms | This study |
| pRGK341 | ccsB gene cloned into pET2-Blue with C-terminal 6 × His tag; Ampr | This study |
| pRGK342 | CcsA replacing PhoA from pRGK309; Tetr | This study |
a
ORF, open reading frame.