TABLE 2.

B. pertussis Tn5 mutants with defects in TMPD oxidase

Straina	Gene with Tn5 insertion	Location of Tn5 in geneb	TMPD oxidase activityc	% of cytochrome c producedde	Synthesis of cytochromes corrected by DTTef
CB08	ctaC (CoxII)	G217→	—	NR	NR
CB01	ctaD (CoxI)	G247→	—	NR	NR
CB27	ctaD	V95←	—	NR	NR
CB134	ctaD	G87→	—	NR	NR
CB116	ctaE (CoxIII)	A270←	—	NR	NR
CB28	ctaB	N195→	—	NR	NR
CB114	ctaB	I147→	—	NR	NR
CB106*	sco2	V67→	—	NR	NR
CB15	hemZ (hemH)	Upstream	—	<10	No
CB05	dipZ (dsbD)	E99←	+/−	<50	Yes
CB07	dipZ	L387→	+/−	<50	Yes
CB10	dipZ	P137←	—	<1	Yes
CB26	dipZ	A273→	—	<1	Yes
CB82*	dipZ	G205←	—	<1	Yes
CB128	dipZ	L290→	—	<1	Yes
CB115	dipZ	R82←	—	<1	Yes
CB84*	cycC	P117←	—	<10	No
CB75	ccsA	V132→	—	<1	No
CB83*	ccsA	G43←	—	<1	No
CB121	ccsA	D348→	—	<1	No
CB127	ccsA	D30←	—	<1	No
CB133	ccsB	Y58→	—	<1	No
CB110*	ccsB	S55→	—	<1	No
CB111*	ccsB	V550←	—	<1	No
CB129	ccsB	N501→	—	<1	No
CB130	ccsB	G429→	—	<1	No
CB123	ccsB	Y79←	—	<1	No

^aAn asterisk indicates a strain with a Tn5 insertion in a dsbB background.

^bThe amino acid residue is indicated, followed by an arrow which refers to the direction of the GFP (in the mini-Tn5) with respect to the orientation of the gene.

^cActivity is noted as detected in single colonies and restreaked from each strain. —, not detectable above background; +/−, less than that of the wild type but above background.

^dProfiles of c-type cytochromes are as shown in Fig. 1 and described in the text. The values are relative to that produced by the WT.

^eNR, not relevant, since these mutants have wild-type levels of c-type cytochromes.

^fDTT was added to the growing cultures at various concentrations, and then extracts were assayed for c-type cytochromes, as depicted in Fig. 1.