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. 2005 Jun;187(12):3950–3959. doi: 10.1128/JB.187.12.3950-3959.2005

FIG. 2.

FIG. 2.

(A) RT-PCR analysis of transcription of the traH, traJ, and trhR (Tra1) genes and the trhA and trhE (Tra2) genes from plasmid R27 in WT and hha hns backgrounds. Cells were grown at 25°C (lanes 1 and 2) or 33°C (lanes 3 and 4). Lanes 1 and 3, strain 5K(R27); lanes 2 and 4, strain Hha3-hns(R27 hha hns). (B) RT-PCR analysis of transcription of trhR in cells lacking Hha-like proteins. Cells were grown at 25°C (lanes 1 and 2) and at 33°C (lanes 3 and 4). Lanes 1 and 3, strain 5K(R27); lanes 2 and 4, strain Hha3 ydgT(R27 hha). RT-PCR of 16S rRNA was used as a control to confirm equivalent quantities of template loading.