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Plasmid R27 compensates for hha-hns-induced deregulation of hemolysin expression (B) and for alterations of the growth rate at 37°C (A). The strains tested were 5K(pANN202-312) (squares), Hha3 hns(pANN202-312) (circles), Hha3 hns(pANN202-312 R27) (triangles), and Hha3 hns(pANN202-312 R27 hha hns) (diamonds). Hemolytic activity of strain Hha3 hns(pANN202-312), referred to as 100%, was 7,630 U. OD600, optical density at 600 nm.
