TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| 5K | F−hsdR hsdM thr thi leu lacZ | 23 |
| 5K Rf | 5K Rifr | 37 |
| 5K hns | 5K hns::Ampr | This work |
| Hha3 | 5K hha::Tn5phoA | 17 |
| Hha3 hns | Hha3 hns::Ampr | This work |
| S17-1 λpir | Tpr SmrrecA thi pro hsdR H+ | 31 |
| S614 | CSH50 Δbgl-AC11 ΔlacZ(pro+) hns::Ampr | 8 |
| E. coli R27 | Rifr Tcr | 42 |
| Plasmids | ||
| R27 | IncHI1 Tcr | 18 |
| R27 hha | R27 hha::mini-Tn5Km1 | This work |
| R27 hns | R27 Δhns | This work |
| R27 hha hns | R27 hha::mini-Tn5Km1 Δhns | This work |
| pUTmini-Tn5Km1 | Apr Kmr ori R6K mini-Tn5Km1 | 7 |
| pKD3 | Template plasmid | 5 |
| pKD46 | Red helper plasmid Apr | 5 |
| pCP20 | FLP helper plasmid Apr Cmr | 5 |
| pANN202-312 | hlyC hlyA hlyC hlyD Cmr | 17 |