TABLE 4.

Specific oligonucleotides used in this work for labeling of mRNAs corresponding to ORFs of plasmid R27 and controls

Region and oligonucleotide	Sequence (5′-3′)
Tra 1
TraJ	ACATAAGTTTTTGAAAGTTA
R0118	TTATCCGCAGCCATTTG
TraG	TGAGGCACTTCTGTACT
TraI	AGGCGCATTTAGCTGC
TraH	TCATTGTGCCACCTCTG
TrhR	ATCCTGACGGTGGCAT
TrhY	CTAATCTAACTCTTCCTG
TrhX	CGTTAATCGAGTCATTTAC
TrhF	TATGCACCTCCTAAACTT
TrhH	TGATTGGTCAGGTTTTCA
TrhG	TCATTCAGCCAGCTTTTA
Tra 2
TrhI	TTATTCTGTCAAAGGGGAT
R0004	TTATTTTGATTTCAGAGTTTT
TrhN	ATATTTTGGTATTTCCTTAC
TrhU	TGTAAACATAGTTATCTATG
TrhW	CAATTCTCCTCTGCCG
TrhF	AATAGTTAATTCTCTTTCAAT
R0009	TTTTGCTTCCCACGATT
HtdK	CACTGACCGCTCTTAG
HtdF	CATACTCTCTTCTTAAAGA
HtdA	GCACATAAGCCTGAGTT
TrhO	AATCCAGGTTAAGTGGT
R0016	AATCATTTTCAGTCCTCAT
R0017	AAATGATTGAGTGACTACA
TrhZ	ATCTATATGAACCGCTG
TrhC	CTGATTTGCTCGCAACT
TrhV	AATACCGTTTGACGAAAC
HtdT	AATGGGTTTTTGAGTAAG
R0028	TCAATTATCGAATCTCACT
TrhB	CACTGCTGCTCAGCTA
R0030	ATGTCCATTATTAACTCCA
TrhK	TTATCTTTTAATCAGGCTT
TrhE	TTAGTTACCTATTGACGG
TrhL	CCATCAAGAATAGAGGTT
TrhA	GCATCCAGGAACGAG
Positive controls
rrsH	GGTTCCCCTACGGTA
zwf	ACTCAGCATCGCGCC