TABLE 5.

Effects of hns and hha mutations on temperature-dependent conjugation of R27

Donor strain	Conjugation frequency at:
	25°C	33°C
5K(R27)	2.6 × 10−3	<10−9
5K(R27 hha)	3.6 × 10−3	1.1 × 10−7
5K(R27 hns)	2.8 × 10−3	0.8 × 10−7
5K(R27 hha hns)	3.0 × 10−3	2.7 × 10−6
Hha3(R27)	1.5 × 10−4	5.2 × 10−6
5K hns(R27)	2.0 × 10−2	2.5 × 10−5
Hha3 hns(R27)	4.0 × 10−2	5.0 × 10−5
Hha3 hns(R27 hha hns)	1	1.0 × 10−2