FIG. 1.
Motilities of H. pylori 43504 and various mutant derivatives. Cells were inoculated on semisolid motility agar plates with a sterile toothpick and incubated for 4 to 5 days at 37°C under microaerophilic conditions. Strains that were tested for motility were H. pylori 43504 (wild type), an rpoN:aphA3 mutant, an hp0958:aphA3 mutant, an hp0958:aphA3 mutant in which a functional copy of hp0958 was introduced into the hp0405 locus (hp0958:aphA3/hp0405:hp0958), and H. pylori 43504 bearing the PureA-hp0958 allele in the hp0405 locus (hp0405:PureA-hp0958).