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. 2005 Jul;187(13):4521–4530. doi: 10.1128/JB.187.13.4521-4530.2005

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FIG. 3. — Effects of external phosphatase and cross-feeding on an NAD-limited lawn. The plate contained nadB pncA aphA (TT20738) cells embedded in minimal top agar on top of minimal agar (the agar contained 200 μM NMN, 0.2%glucose, and 1% tryptophan, as described in Materials and Methods). Growth of the lawn was stimulated by added alkaline phosphatase (bottom left) and by a spotted culture of an aphA* mutant (TR7264) and was stimulated slightly by a spotted culture of the wild type (TR5987) or a PnuC* mutant (TR7253).