TABLE 2.
Growth phenotypes with standard and low concentrations of NMN
| Strain(s) | Relevant genotypea | Growth with:
|
|
|---|---|---|---|
| 100 μM NMN | 10 μM NMN | ||
| TT13007 | nadB pncA | + | − |
| TT24215 | nadB pncA pnuC | − | − |
| TT14946 | nadB pncA nadR(T−) | − | − |
| TT20738, TT22890 | nadB pncA aphA | − | − |
| TT22458 | nadB pncA aphA/pBAD(aphA) | + | − |
| TR7262-TR7264 | nadB pncA aphA* | + | + |
| TT22911 | nadB pncA aphA* nadR(T−) | − | − |
| TT24642 | nadB pncA aphA* pnuC | − | − |
| TR7253-TR7261 | nadB pncA pnuC* | + | + |
| TT24639 | nadB pncA pnuC* aphA | + | + |
| TT22897 | nadB pncA pnuC* nadR(T−) | + | + |
| TT15564 | nadA pncA pnuC pnuD* | + | − |
| TT22947 | nadA pncA pnuC pnuD* aphA | − | − |
| TT22943 | nadA pncA pnuC pnuD* nadR(T−) | − | − |
a
Full genotypes are shown in Table 1. All pnuC mutations are all null types except pnuC* which allows uptake of intact NMN as well as NmR. Phenotypes indicated in parentheses are as follows: nadR(T−), deficient in use of NMN; aphA*, increases the level of AphA; pnuD*, allows an unrelated transporter to import NmR.