TABLE 1.
Bacterial strains, plasmids, and phages used in this study
| Strain plasmid, or phage | Property | Reference or source |
|---|---|---|
| Strains | ||
| E. coli XL-1 Blue | Expression of srtAΔN | 8 |
| E. coli DH5α | Plasmid transformation | 21 |
| E. coli K1077 | dam dcm mutant, plasmid purification for B. anthracis transformation | P. Model/M. Russel collection |
| B. anthracis Sterne 34F2 | Vaccine strain, pXO1+ and pXO2− | 56 |
| B. anthracis AHG188 | ermC replacement of srtA (BA0688/BAS0654) in strain Sterne | This work |
| B. anthracis AHG263 | Δ(srtA)::ermC CP-51 transduced into strain Sterne | This work |
| Plasmids | ||
| pOS1 | Multicopy shuttle vector | 52 |
| pAHG279 | Full-length srtA cloned in pOS1 | This work |
| pAHG277 | basCFLAG/MH6 cloned in pOS1 | This work |
| pAHG322 | basCFLAG/MH6 and srtA cloned in pOS1 | This work |
| pTS1 | Temperature-sensitive shuttle vector | 36 |
| pAHG107 | 5′-srtA-ermC-3′-srtA cassette in pTS1 | This work |
| pQE30 | Vector for expression cloning of His6 fusions | Qiagen |
| pAHG316 | srtAΔN cloned in pQE30 | This work |
| Phage | ||
| CP-51 | B. anthracis transducing phage | 20 |