. 2005 Jul;187(13):4607–4614. doi: 10.1128/JB.187.13.4607-4614.2005

TABLE 1.

Genes mutated in this study

Gene^a	Known or predicted function	C₁ growth defect^b
mxaE1^*	Unknown function in methanol oxidation	No*
mxaD1^*	Methanol oxidation, interaction between methanol dehydrogenase and cytochrome c_L	No^*
mxaD2^*	Methanol oxidation, interaction between methanol dehydrogenase and cytochrome c_L	No^*
pabB^*	Component 1 of PABA synthase, biosynthesis of H₄F and H₄MPT	Yes^*
pcbD^*	Pterin-4a-carbinolamine dehydratase, regeneration of BH₄	No^*
pts^*	6-Pyruvoyl tetrahydropterin synthase, biosynthesis of BH₄	No^*
fae2^*	Formaldehyde-activating enzyme	No^*
fae3^*	Formaldehyde-activating enzyme	No^*
fae2 fae3		No^*
orf1^*	ATP-utilizing protein, ATP grasp family	Yes^†
afpA^*	Archaeal flavoprotein, FMN-binding electron transfer protein	Yes^†
orf5^†	Unknown function in biosynthesis of H₄MPT	Yes^†
mtdB^†	Methylene-H₄MPT dehydrogenase	Yes^†
mptG^†	Ribofuranosylaminobenzene 5-phosphate synthase, biosynthesis of H₄MPT	Yes^†
orfY^*	Predicted ATP-dependent carboligase	Yes^†

*, Function is predicted based on sequence similarity; †, function has been characterized in M. extorquens. See text for references.

*, Mutated in M. extorquens; †, mutated in M. flagellatus.