TABLE 2.
Growth with heme or hemophore as the sole porphyrin sourcea
| Strain | Growth
|
||||
|---|---|---|---|---|---|
| Heme (μM)
|
Holo-HasA (μM)
|
||||
| 10 | 5 | 1 | 10 | 1 | |
| C600 ΔhemA(pBAD24-hasR) | ++ | ++ | + | +++ | ++ |
| C600 ΔhemA(pBAD24-hasR Δ11-91) | ++ | ++ | + | +++ | ++ |
| C600 ΔhemA(pBAD24-hasR Δ11-192) | + | + | − | − | − |
| C600 ΔhemA(pBAD24-hasR Δ11-192 + pBAD33-hasR 1-242) | + | + | − | − | − |
| C600 ΔhemA-exbB::Tn10(pBAD24-hasR) | − | − | − | − | − |
| C600 ΔhemA-exbB::Tn10(pBAD24-hasR Δ 11-91) | − | − | − | − | − |
| C600 ΔhemA-exbB::Tn10(pBAD24-hasR Δ 11-192) | + | + | − | − | − |
| C600 ΔhemA hemA-exbB::Tn10(pBAD24-hasR Δ11-192 + pBAD33-hasR 1-242) | + | + | − | − | − |
| C600 ΔhemA(pBAD24-hasR Δ11-192 H603A) | − | − | − | − | − |
| C600 ΔhemA-exbB::Tn10(pBAD24-hasR Δ11-192 H603A) | − | − | − | − | − |
a
Growth was measured around 5-mm wells filled with 50 μl of the indicated solution. −, no growth; +, halo of growth around the well with a radius of 1 mm; ++, halo of growth around the well with a radius of 5 mm; +++, halo of growth around the well with a radius of 10 mm.