TABLE 3.
Effect of HasR 1-242 on growth
| Strain | Growth
|
|||
|---|---|---|---|---|
| Heme (μM)
|
Holo-HasA (μM)
|
|||
| 10 | 1 | 10 | 1 | |
| C600 ΔhemA-att λ::hasR(pBAD24, pACYC184) | ++ | + | +++ | ++ |
| C600 ΔhemA-att λ::hasR(pBAD24-hasR 1-242, pACYC184) | ++ | + | − | − |
| C600 ΔhemA-att λ::hasR(pBAD24 hasR 1-242 + pNC1) | ++ | + | +++ | ++ |
a
Growth was measured around 5-mm wells filled with 50 μl of the indicated solution. −, no growth; +, halo of growth around the well with a radius of 1 mm; ++, halo of growth around the well with a radius of 5 mm; +++, halo of growth around the well with a radius of 10 mm.