TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s)a | Sourceb or reference |
|---|---|---|
| Strains | ||
| Synechococcus sp. strain WH8102 | Motile strain, recipient in conjugations with pRL27 and pMUT100 constructions | J. Waterbury |
| E. coli | ||
| MC1061 | Host for pRK24, pRL528; used as a donor in pJM construction conjugations | 8 |
| DH5α | Recipient in transformations | BRL |
| BW20767 | Used as donor for pRL27 conjugations | 21 |
| Transformax EC100D pir+ | Expresses pir gene product for propagation of vectors containing the R6Kγ origin of replication | Epicentre |
| Plasmids | ||
| pMUT100 | Kanr, Tetr; suicide vector | 5 |
| pRL27 | Kanr; mini-Tn5 plasposon (oriR6K) delivery vector | 15 |
| pRK24 | Tcr, Ampr; conjugal plasmid, RK2 derivative | 5, 22 |
| pRL528 | Cmr; helper plasmid, carries mob | 5, 10 |
| pCR2.1-TOPO | Kanr, Ampr; PCR product cloning vector | Invitrogen |
| pJM37 | pMUT100 containing SYNW0079 fragment of nt 78600 to 78939 | This work |
| pBB1000 | pMUT100 containing swmA fragment of nt 84507 to 84926 | This work |
| pJM62 | pMUT100 containing SYNW0087 fragment of nt 88793 to 89004 | This work |
| pJM18 | pMUT100 containing SYNW0088 fragment of nt 89414 to 89635 | This work |
| pJM59 | pMUT100 containing SYNW0192 fragment of nt 193443 to 193661 | This work |
| pJM40 | pMUT100 containing SYNW0193 fragment of nt 194020 to 194287 | This work |
| pJM20 | pMUT100 containing swmB fragment of nt 913153 to 913396 | This work |
| pJM35 | pMUT100 containing SYNW0957 fragment of nt 947811 to 948110 | This work |
| pJM34 | pMUT100 containing SYNW0960 fragment of nt 953811 to 954171 | This work |
a
nt, nucleotide. Numbering according to reference 23.
b
BRL, Bethesda Research Laboratories, Gaithersburg, MD; Invitrogen, Carlsbad, CA; Epicentre, Madison, WI.