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Structure of the plasmids used in this study. Expression of the repN and mutant repN genes are under the control of the spac promoter induced by IPTG. The dotted arrow in pHDMAE21 indicates the mutant repN gene containing sequence alterations in all five iterons. Abbreviations: Ac, AccI; Ba, BamHI; Bn, BanIII; Bp, BspEI; Bs, BstXI; Ec, EcoRI; Hi, HindIII; Ps, PstI; Sm, SmaI; Xb, XbaI.
