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Amplification of the copy number of pHDCS2 by overexpression of the repN gene. Experimental procedures are described in Materials and Methods. Lane 1, λ DNA digested with HindIII; BamHI digests of total DNA isolated from the cells grown in the presence of IPTG at concentrations of 0 mM (lane 2); 0.008 mM (lane 3); 0.04 mM (lane 4); 0.2 mM (lane 5); 1.0 mM (lane 6).
