FIG. 8.

Incompatibility (A) and replication ability (B) shown by various regions of repN. The coding region of repN depicted in the box is from nt 6256 to 7116 with iterons (arrows) located from nt 6686 to nt 6810. The map is not drawn to scale. The small boxes indicate the A+T-rich region in the repN-coding region as defined in the legend to Fig. 2. (A) The host strains (ISHI11-ISHI34) carrying the respective regions at the amyE locus were made competent, and transformed with pSEQ243. ++, +, and − indicate strong, intermediate, and no incompatibility activities, respectively (for definitions, see the text). (B) Replication ability of pHDMAE21 carrying the DNA regions shown. + and − indicate the colony forming abilities of the plasmids on Cm-containing plates when transformed into CU741.