TABLE 3.
Transport ability of NadR RNK point mutations in H. influenzaea
| Strain | Mutation | Uptake of 14C label (%) | SD |
|---|---|---|---|
| SE01 | Wild type | 65.1 | 6.250 |
| SE01ΔRNK | ΔRNK | 3.1 | 0.565 |
| SE02ΔRNK | NadR-His6 | 70.0 | 7.587 |
| SE03ΔRNK | G238N | 4.8 | 2.375 |
| SE04ΔRNK | G238S | 5.8 | 0.799 |
| SE05ΔRNK | D304C | 16.3 | 2.354 |
| SE06ΔRNK | D304N | 19.3 | 0.289 |
| SE07ΔRNK | D304S | 4.5 | 3.394 |
| SE08ΔRNK | R352C | 4.4 | 1.237 |
| SE09ΔRNK | R352M | 3.4 | 1.414 |
| SE10ΔRNK | R352N | 6.0 | 0.424 |
a
The values indicate the accumulation of [14C]NAD+-derived substrate for strains SE01ΔRNK to SE10ΔRNK (Table 1) having point mutants in nadR. The uptake of the 14C label indicates the intracellular accumulation of cpm derived from [14C]NAD provided in the growth medium (100%). The standard deviations are based on three independent measurements.