TABLE 2.
Primer sequences used for PCRa
| Name | Sequence (from 5′ to 3′) | Description |
|---|---|---|
| M13 reverse | CAGGAAACAGCTATGACC | Sequencing |
| M13 universal | GTTGTAAAACGACGGCCAGT | Sequencing |
| RHI-168 | CCGAATTCAGGGCGCCTATGATATTGAAATC | Putative spi4_H promoter region |
| RHI-169 | GGCTGCAGTTAACGTGTAGCTGCCATCCGCC | Putative spi4_H promoter region |
| RHI-184 | CTGACTCTCTCTGCATCAGGATATACGGCAG | Site-directed mutagenesis hilA |
| RHI-185 | CTGCCGTATATCCTGATGCAGAGAGAGTCAG | Site-directed mutagenesis hilA |
| RHI-186 | GGGTTTAATAACTGCACCAGATAAACGCAGTCG | Site-directed mutagenesis sicA |
| RHI-187 | CGACTGCGTTTATCTGGTGCAGTTATTAAACCC | Site-directed mutagenesis sicA |
| PRO-407 | TTAACCATGGCTCATTTTAATCCTGTTCC | Forward hilA in pCMPG5338 (59) |
| PRO-408 | TTGTTCTAGAATTAATTTAATCAAGCGGGG | Reverse hilA in pCMPG5338 (59) |
a
Restriction sites are indicated in bold; mutated positions are underscored.