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Gel analysis of the dOgg1 purification steps. After expression in E.coli the purification fractions were analyzed by SDS–PAGE. M, molecular weight markers; lane 1, total cell lysate; lane 2, eluate from the glutathione–Sepharose 4B; lane 3, products of the thrombin cleavage reaction; lane 4, eluate from the MonoS column.
