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. 2000 Dec 1;28(23):4583–4592. doi: 10.1093/nar/28.23.4583

Figure 4.

Figure 4

Cleavage activity of dOgg1 on DNA duplexes harboring single lesions. (A) Cleavage of 34mer oligodeoxyribonucleotides carrying a single 8-OH-Gua paired to each of the four normal DNA bases. (B) Cleavage of 34mer oligodeoxyribonucleotides carrying a single 8-OH-Ade paired to each of the four normal DNA bases. (C) Cleavage of 34mer oligodeoxyribonucleotides carrying a single AP site paired to each of the four normal DNA bases. The DNA duplexes were 32P-labeled at the 5′-end of the strand carrying the lesion. Incubations were performed at 37°C for 15 min. The products were separated on 20% PAGE containing 7 M urea and quantified using a PhosphorImager.