. 2000 Dec 1;28(23):4583–4592. doi: 10.1093/nar/28.23.4583

Table 1. Kinetic constants for excision of 8-OH-Gua and FapyGua by Drosophila Ogg1 protein from DNA γ-irradiated under N₂O.

Lesion	V_max (nM min^–1)^a	K_M (nM)^a	k_cat × 10³ (min^–1)^a	k_cat/K_M × 10⁵ (min^–1 nM^–1)^a
8-OH-Gua	15.0 ± 0.3	267 ± 16^b	56.2 ± 1.1	21.0 ± 0.4^b
FapyGua	12.7 ± 0.2	422 ± 22	47.5 ± 0.7	11.2 ± 0.2

^aValues represent the mean ± standard deviation (n = 6). k_cat = V_max/[enzyme]: ([enzyme] = 267 nM). The concentration ranges of the FapyGua and 8-OH-Gua were 0.82 – 3.8 µM, and 0.41 – 2.3 µM, respectively.

^bStatistically different from the value in line 2 (P < 0.05).

Table 1. Kinetic constants for excision of 8-OH-Gua and FapyGua by Drosophila Ogg1 protein from DNA γ-irradiated under N2O.

Table 1. Kinetic constants for excision of 8-OH-Gua and FapyGua by Drosophila Ogg1 protein from DNA γ-irradiated under N₂O.