FIG. 7.

Interaction of RapF with ComA and PhrF. ComA (10 μM), RapF (10 μM), and PhrF (50 μM) were analyzed on a 10% Tris-Tricine-EDTA native gel stained with Coomassie blue. The gel was run at 4°C for 24 h at constant voltage (63 V). In lane 4, RapF was preincubated with ComA for 5 min before the addition of PhrF followed by a further 5-min incubation at room temperature. In lane 5, RapF was preincubated with PhrF and then ComA was added to the reaction mixture for a further 5-min incubation.