TABLE 1.
Bacillus subtilis strains used in this study
| Strain | Relevant genotypea | Originb |
|---|---|---|
| JH642 | Parental | Laboratory stock |
| JH646 | spo0A12 | Laboratory stock |
| JH651 | spo0H81 | Laboratory stock |
| JH12547 | spo0A12, abrB::Tn917mls | Laboratory stock (36) |
| JH12575 | abrB::Tn917mls | Laboratory stock (36) |
| JH12981 | amyE::rapA-lacZ aph3A | Laboratory stock |
| JH23059 | amyE::rapA-lacZ aph3A rapF::cat | pRapF37→JH12981 |
| JH23065 | amyE::rapA-lacZ aph3A rapC::spc rapF::cat | JH11196→JH23059 |
| JH11030 | amyE::rapA-lacZ aph3A rapC::cat | JH12981→JH12939 |
| JH11204 | amyE::rapF-lacZcat | pRapF32B→JH642 |
| JH11208 | amyE::rapF-lacZspc | pCm::Spc→JH11204 |
| JH11223 | comA::cat amyE::rapF-lacZspc | BD1626→JH11208 (47) |
| JH11224 | comP::cat amyE::rapF-lacZspc | BD1658→JH11208 (47) |
| JH11423 | amyE::rapA-lacZ aph3A phrC::spc | JH12981→JH11303 |
| JH11500 | amyE::rapA-lacZ aph3A phrF::cat | JH11478→JH12981 |
| JH11507 | amyE::phrF-lacZcat | pRapF38→JH642 |
| JH11508 | amyE::rapA-lacZ aph3A phrC::spc phrF::cat | JH11478→JH11423 |
| JH19163 | amyE::rapFphrF-lacZcat | pRapF39→JH642 |
| JH23060 | amyE::rapA-lacZ aph3A pSS8 | pSS8→JH12981 |
| JH23061 | amyE::rapA-lacZ aph3A pSS9 | pSS9→JH12981 |
| JH23062 | amyE::rapA-lacZ aph3A pSS10 | pSS10→JH12981 |
| JH23063 | amyE::rapA-lacZ aph3A pSS11 | pSS11→JH12981 |
| JH23064 | amyE::rapA-lacZaph3A pHT315 | pHT315→JH12981 |
| JH23066 | spo0A12 amyE::phrF-lacZcat | JH11507→JH646 |
| JH23067 | spo0A12 abrB::Tn917mls amyE::phrF-lacZcat | JH11507→JH12547 |
| JH23068 | abrB::Tn917mls amyE::phrF-lacZcat | JH11507→JH12575 |
| JH23069 | spo0H81 amyE::phrF-lacZcat | JH11507→JH651 |
| JH11196 | rapC::spc | pCm::Spc→JH12939 |
| JH12939 | rapC::cat | p0M47→JH642 |
| JH11298 | phrC::cat | p0M88→JH642 |
| JH11303 | phrC::spc | pCm::Spc→JH11298 |
| JH11478 | phrF::cat | pRapF35→JH642 |
a
All strains are derivatives of JH642 and thus carry the trpC2 phe-1 auxothrophic markers. Antibiotic resistance genes are expressed as follows: mls, erythromycin and lincomycin; aph3A, kamamycin; cat, chloramphenicol; spc, spectinomycin.
b
Arrows indicate construction by transformation using plasmid DNA or chromosomal DNA.