FIG. 6.

JD614 membranes release less polysaccharide than WU2 membranes. Polysaccharide-enzyme complexes were synthesized as described in Materials and Methods. Labeled complexes (10,000 cpm for WU2 and 15,000 cpm for JD614) were incubated with either 100 μM UDP-Glc (solid bars) or 100 μM UDP-GlcUA (open bars), and polysaccharide release from the membranes was assayed as described previously (15). The data presented are averages (± standard deviations) for duplicate release assays using the same polysaccharide-enzyme complex. The amount of release observed with no nucleotide sugar (5% for WU2 and 2% for JD614) was subtracted from each sample prior to averaging data.