TABLE 1.
Phosphorous and hexose levels during lipid purification
| Fraction or process | Result for strain
|
|||||
|---|---|---|---|---|---|---|
|
S. pneumoniae JD614
|
E. coli JD424
|
|||||
| Amt of hexosea (μmol) | Amt of phosphorousa (μmol) | Hexose/phosphorous ratio | Amt of hexosea (μmol) | Amt of phosphorousa (μmol) | Hexose/phosphorous ratio | |
| Chloroform extract | 8 | 2 | 4 | 2.3 | 74.5 | 0.03 |
| Anionic lipids | 0.2 | 1.2 | 0.17 | 1.8 | 14.5 | 0.12 |
| TLCb | 0.086 | 0.076 | 1.2 | 1 | 0.8 | 1.25 |
a
Aliquots of the fractions indicated were measured for total hexose and phosphorous content as described in Materials and Methods. These values were used to determine the hexose and phosphorous content in the total fraction, which is indicated.
b
Lipid band purified by thin-layer chromatography.