FIG. 2.

Growth inhibition of E. coli Frag1 by Hcy. (Top) Cultures of Frag1 were grown in MM (0.4% [wt/vol] glucose) and supplemented with 0 (control), 0.2, 0.5, 2.0, 8.0, or 20.0 mM Hcy. Growth was monitored by recording the OD₆₀₀ at suitable time intervals after inoculation. (Bottom) Frag1 was inoculated into MM (0.4% [wt/vol] glucose) supplemented with 1 mM Hcy at a starting optical density (OD₆₀₀) of 0.128 (▵), 0.047 (▴), or 0.057 (◊). The control culture (▪) had no added Hcy and had a starting OD₆₀₀ of 0.022. Vertical dashed lines indicate the time points that Hcy-treated cultures showed a transition from slow inhibited growth to faster growth rates.