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Amino acid pools are perturbed in the presence of Hcy. Cultures of Frag1 were grown in MM with 0.4% glucose and grown to an OD600 of 0.2. Amino acid pools were analyzed 60 min after either no addition (□), the addition of 0.5 mM Hcy (░⃞), or the addition of 0.5 mM Hcy plus 0.15 mM Ile (▪) as described in Materials and Methods. Error bars indicate the standard deviation from the mean (n = 4).
