FIG. 8.

Reverse transcriptase PCR (RT-PCR) analysis of the transcription of ilvA. cDNA concentrations were normalized by using primers against polA. RNA was prepared from cells grown in the presence (+Hcy) or absence (−Hcy) of 0.5 mM Hcy. Hcy was added to cultures at an OD₆₀₀ of ∼0.2 and incubated for 1 h before RNA extraction. The DNA products obtained after 30 cycles of RT-PCR are shown.