TABLE 1.
PCR primers used in this study
| Primer | Sequence (5′-3′)a |
|---|---|
| ilvAFOR1 | CACCTAGAGGAGATATACATACCCATGGCTGACTCGCAACCCCTGb |
| ilvAREV1 | CTAGTGATGGTGATGGTGATGACCCGCCAAAAAGAACCTGAACGc |
| ilvAFOR2 | CCGCAAATCAAAGTGATC |
| ilvAREV2 | GAATTCGAAGCTGTAGAG |
| polAFOR | GAAGAGTTGTTGGGGCTG |
| polAREV | CAGACGAAGGGTGAGCTC |
a
Primers were designed by using published sequences from the Colibri website (http://genolist.pasteur.fr/Colibri/).
b
Underlined sequence is the start site in ilvAFOR1.
c
Underlined sequence is the 3′ His tag in ilvAREV1.